IDENTIFICATION AND ANTIBIOTIC SUSCEPTIBILITY PATTERN OF WOUND ISOLATES IN SCHEER MEMORIAL HOSPITAL 

Abstract

Wound is the most common site for the bacterial infection. Antibiotic treatment was
considered to be the important method of treatment, but the treatment has been
compromised due to emergence of organisms that are resistant to multiple antibiotics.This
study was carried out in the microbiology laboratory of Scheer memorial hospital,
Banepa, Nepal, with the aim to determine the causative agents of wound infection and
their antibiotic susceptibility pattern.
A total 112 swabs and 97aspirates were processed in the period of 9 months (April-
December 2010 AD). Samples from male patients were found more in number (54.45%,
n=114) as compared to female patients (45.45%, n=95). The age ranged from 2 months to
91 years where most susceptible age group was 20-30 (25.36%).Bacterial growth was
found in 150 (71.77%) samples out of which138 (92%) showed single growth and 12(8%)
showed multiple isolates. Specimens in swab showed more mixed growth (10.98%) as
compared to the Aspirates (4.41%). Total 162 species of aerobic bacterial species were
isolated from 150 samples and out of which 99(61.11%) were gram positive and
63(38.89%) were gram negative organisms. S. aureus was the most predominant species.
Antibiotic Susceptibility test showed that Gentamicin could be the drug of choice for
gram positive wound isolates which was 82.83% sensitive and Amikacin was the most
effective drug for gram negative isolates which was 84.12% sensitive. Out of 77 S.
aureus, 46(59.74%) were Methicillin Resistant S. aureus (MRSA) and all (100%) were
sensitive towards Vancomycin.