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STUDY OF DIFFERENT BACTERIAL AGENT IN THE CASES OF ACUTE CONJUNCTIVITIS AND ITS ANTIBIOTIC SENSITIVITY. 

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Resource details

Resource ID

237

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Contributed by

Rajan Karmacharya

Author/Contributor

Pratima Thapa

Publisher/Credit

St. Xavier's College

Date

16 November 16

Document type

Thesis or project

Department

Microbiology

Level

Master

Batch

2008

Abstract

This study of conjunctivitis was carried out based on the patients attending B.P Koirala Lions Center for Ophthalmic Studies, during 10th April 2010- 23rd August 2010. This study aims to have a thorough analysis of the bacterial agents present in the eyes of the conjunctivitis patients. A total of three hundred and fifty eye patients from the Kathmandu valley and its surrounding were examined. The result shows that out of 108 positive samples, 72 organisms (66.66%) were from male patients whereas 36 organisms (33.34%) were from female patients (P<0.05). Among the growth positives, 96 samples were found to be Gram positive whereas 12 samples were Gram negative. 67 samples out of 96 numbers of grams positive were from the male patients while 29 samples were from the female patients. Similarly out of 12 Gram negative samples, 9 samples were from male patients and 3 samples were from female patients. Among 108 positive samples, coagulase negative Staphylococcus sps (CONS) were more dominant and observed in 54 samples. Then followed by Staphylococcus aureus which were found in 26 sample and Streptococcus pneumonia in 16 samples respectively. Haemophilus influenza were isolated in 8 samples, where as Pseudomonas aeruginosa and E. coli were least isolated in 2 samples each. The most common microbial pathogen causing conjunctivitis was CONS (P>0.05). The children were seen more susceptible to the bacterial conjunctivitis. Nevertheless, positive isolates were seen in all age groups of adults. The antibiotic sensitivity pattern reveals that out of 96 Gram positive isolates chloremphenicol was found to be most sensitive in 76.04% of the total positive growth followed by Cephalexin (71.88%) and ciprofloxacin (66.67%). Cephazoline, Gentamycin, Olfloxacin, and Tobramycin were found to be sensitive only in 53.13%, 52.08%, 48.96% and 45.83% respectively. Among these 12 gram negative isolates, Ciprofloxacin was most effective drugs (75%), Chloremphenol (66.66%), Cephalexin (58.33%), chloremphenicol (66.66%) while Gentamycin (41.66%), Olfloxacin (33.33%), and Tobramycin (25%) were found to be less effective. Finally, the research data shows that a large number of people are affected by the conjunctivitis in our country. It is very essential for an intensive study nationwide about the identification, cause, prevention, mode of transmission and treatment of conjunctivitis which will be very beneficial to protect our vital organ eyes.

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